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. 2020 May 19;13:261. doi: 10.1186/s13071-020-04131-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — 18S rDNA sequences placed in GenBank and identified as Theileria equi but detected within various host species were gathered and aligned in CLC Genomics Workbench v.10.1.1 (Qiagen), along with outgroup 18S rDNA sequences from other piroplasmids as identified in the figure. All sequences were trimmed to identical length of 401 bp, and span the 4th hypervariable region of full-length 18S rDNA. Aligned sequences were compared in a maximum likelihood phylogeny in the same program, with a Kimura 80 nucleotide substitution model, with neighbor-joining used in constructing initial trees to enable enhanced evolutionary inference. Five hundred replicates were performed in a bootstrap analysis with the values for nodes above a cut-off of 50 indicated on the tree for major branches. The tree provides a representative sample of ribosomal sequences falling into distinct clades across different infected host species and is not is intended to provide an exhaustive depiction of the relationships of all equid Theileria isolates