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Gene expression analysis of Hedgehog pathway components upon treatment with compounds and GlaB. Quantitative PCR (qPCR) analysis of GLI1, PTCH1, HIP1, GLI2, and Cyclin D1 in A375 cells treated with DMSO, SST0682, SST0683, SST0704, and SST0790 or GlaB (5 μM) for 48 h. The y axis represents expression ratio of gene/(HPRT and EIF2α average), with DMSO control equated to 1. Data shown as mean ± SD.
