Figure 8.
Silencing of GLI1 and GLI2 mitigates the effect of SST0683 and SST0790 on melanoma cell viability. (A) Western blot analysis of GLI1 and GLI2 in A375 cells transduced with LV-c, LV-shGLI1, or LV-shGLI2. HSP90 was used as loading control. (B–D) Effect of SST0683 (B) and SST0790 (C) on viability of A375 melanoma cells transduced as indicated. GlaB was used as a reference compound (D). Cells were treated with DMSO and compounds at the indicated doses for 72 h. Data are shown as mean ± SD of at least three independent experiments.