Table 1. T_m Detected for the Signal of Ligand-Bound RT and Shift (ΔT_m) with Respect to the Apo Form of the Enzyme.

Ligand	Tm (°C)a	ΔTm (°C)b
RT WT	54.6 ± 0.2
TSAO	53.2 ± 0.3	–1.4
MAS4	52.0 ± 0.3	–2.6
1	40.1 ± 0.2	–15.6
2	39.6 ± 0.3	–15.0
3	41.0 ± 0.2	–13.6
4	41.0 ± 0.7	–14.5
5	38.8 ± 1.1	–15.8
6	38.8 ± 0.5	–15.8

^aT_m (−dI/dT max).

^bShift in HIV-1 RT melting temperature measured in the presence of ligand. T_m values are the average of three independent measurements.