Table 4. P-gp Inhibition in an MDR Cancer Cell Line.
| treatments | MFIa | FARb | SIc |
|---|---|---|---|
| NCI-H460 controld | 134.10 | ||
| NCI-H460/R controld | 16.96 | ||
| Dex-VERe | 36.07 | 2.13 | 26.90 |
| 4 | 28.97 | 1.71 | 21.60 |
| 5 | 35.54 | 2.10 | 26.50 |
a
Measured mean fluorescence intensity of Rhodamine 123 (MFI) was used for the calculation of the fluorescence activity ratio (FAR).
b
Via the following equation: FAR = MFIMDR treated/MFIMDR control. FAR values above 1.50 indicate P-gp inhibition.
c
Sensitivity index (SI) was calculated on the basis of the measured mean fluorescence intensity (MFI) expressed via the following equation: SI = (MFIMDR treated × 100)/MFI sensitive control. SI values above 20 account for P-gp inhibition.
d
Sensitive cancer cell line and its MDR counterpart used in the study: nonsmall cell lung carcinoma-NSCLC (NCI-H460 and NCI-H460/R).
e
Dex-VER was applied at the same concentration (20 μM) as tested compounds.