Table 3. Binding Assays and MTT Viability Test on MCF-7 for Compounds 22a,b, 23a,b, and 5.
|
Ki ± SDa (nM) |
IC50 ± SD (μM)b | ||
|---|---|---|---|
| compd | σ1 | σ2 | MCF-7 |
| 5 | >10000c | >10000c | >100d |
| 22a | >10000c | >10000c | >100d |
| 22b | >10000c | >10000c | >100d |
| 23a | 711 ± 127 | 2600 ± 730 | >100d |
| 23b | 62 ± 3 | 127 ± 16 | 87 ± 0.3 |
a
Each value is the mean ± SD of at least two experiments performed in triplicate.
b
Each value is the mean ± SD of at least two experiments performed in quadruplicate.
c
Radioligand displacement lower than 50% at 10 μM.
d
Cell viability reduction lower than 50% at 100 μM.