Table 2. Dissociation Equilibrium Binding Constant KD for Compounds CM03, MM41, and SOP1812 with Quadruplex Sequencesa.
|
G4-hTERT,KD(nM) |
G4-HTR,KD(nM) |
G4 TERRA,KD(nM) |
||||
|---|---|---|---|---|---|---|
| SPR | Fluorescence | SPR | Fluorescence | SPR | Fluorescence | |
| CM03 | 12 ± 2 | 16 ± 2 | 82 ± 6 | 94 ± 5 | 4 ± 1 | 3 ± 1 |
| MM41 | 2 ± 1 | 4 ± 1 | 5 ± 1 | 10 ± 2 | 3 ± 1 | 2 ± 1 |
| SOP1812 | 4 ± 1 | 4 ± 1 | 28 ± 2 | 29 ± 2 | 3 ± 1 | 2 ± 1 |
a
The steady-state binding result obtained as a function of free ligand concentration was fitted to a one-site binding model to obtain KD values.