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. 2020 Mar 30;11(5):913–920. doi: 10.1021/acsmedchemlett.9b00665

Figure 4.

Figure 4

Effect of 1-Glc and Ac-1-Glc compared to PF-543 in TGFβ1-induced fibrosis marker expression in murine myoblasts. C2C12 myoblasts were seeded to 80% confluence and treated with 5.0 ng/mL TGFβ1, PF-543, 1-Glc, and Ac-1-Glc (30 min, 120 min). (A) The expression of protein markers, which expression correlates with muscle fibrosis, has been evaluated by Western blot analysis, using specific anti-α-SMA and anti-SMα22. A representative blot is shown among three independent experiments with analogous results. (B and C) Densitometric analysis was performed of three independent experiments and data, normalized to β-actin expression, were reported as mean ± SD of fold change above control untreated. The effect of pharmacological inhibition of SK1 on TGFβ1 induced fibrosis marker expression was significant by two-way ANOVA followed by Bonferroni posthoc test. #P < 0.05, ##P < 0.01, ###P < 0.001. The effect of pharmacological inhibition of SK1 by 30 min Ac-1-Glc compared to 1-Glc on TGFβ1 induced αSMA expression was significant by two-way ANOVA followed by Bonferroni posthoc test. §§P < 0.01.