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. 2020 May 19;39:91. doi: 10.1186/s13046-020-01594-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Selection of circ-001971 (a) Pathological characteristics of the tumor and non-cancerous tissues revealed by H&E staining. b The expression of the top 11 upregulated circRNAs reported by the previous study was verified in 13 paired tumor and noncancerous tissues using real-time PCR. The data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01. c The expression of circ-001971 was examined in 70 paired tumor and noncancerous tissues using real-time PCR. d Circ-001971 expression was analyzed according to the TNM stage. e ROC curve of circ-001971 to distinguish colorectal cancer (CRC) from controls. f Colorectal cancer (CRC) cases were divided into two groups according to circ-001971 expression. Overall survival was analyzed by Kaplan-Meier overall survival analysis using the log-rank test. g ROC curve of circ-001971 to assess the diagnostic sensitivity and specificity of circ-001971 for CRC