Table 3.
Cosegregation of RYMV resistance and allelic state on CPR5–1
| Phenotype and genotype | ||||
|---|---|---|---|---|
| F2 population | Total | rymv2-Rx | Htz | Rymv2-S1 |
| Og133 (rymv2-R2) x Og431 (Rymv2-S1) | 11 R, 50S (p = 0,23) | 10 R | 22 S | 3 S |
| Og133 (rymv2-R2) x Tog7291 (rymv2-R1) | 55 R, 1S | |||
| Og183 (rymv2-R3) x Og82 (Rymv2-S1) | 14 R, 36 S (p = 0,62) | 14 R | 26 S | 10 S |
| Og213 (rymv2-R4) x Og82 (Rymv2-S1) | 13 R, 42 S (p = 1) | 13 R | 31 S | 11 S |
| Og213 (rymv2-R4) x Tog7291 (rymv2-R1) | 100 R | |||
| Og491 (rymv2-R5) x Og431 (Rymv2-S1) | 24 R, 47 S (p = 0,10) | 21 R | 3 R, 30 S | 17 S |
| Og491 (rymv2-R5) x Tog7291 (rymv2-R1) | 56 R | |||
| Og256 (rymv2-R6) x Og82 (Rymv2-S1) | 18 R, 52 S (p = 0,89) | 18 R, 2S | 37 S | 13 S |
| Og256 (rymv2-R6) x Tog7291 (rymv2-R1) | 45 R | |||
F2 plants were evaluated for RYMV resistance based on symptom observations. The phenotype is indicated with R for resistant plants and S for susceptible ones. For populations derived from crosses with Og82 and Og431 susceptible accessions, the observed phenotypic segregation was compared to a 1R/3S segregation ratio, based on a binomial exact test. For these populations, genotyping on the CPR5–1 gene was generally performed on all plants with CAPS or dCAPS markers, except for the (Og133 x Og431) population for which genotyping was based on Sanger sequencing and only performed on a subset of 35 plants. The genotype is indicated as “rymv2-Rx” for plants homozygous for alleles rymv2-R2 to -R6, “Rymv1-S1” for plants homozygous for the Rymv1-S1 allele, and Htz for heterozygous plants. F2 plants derived from crosses with Tog7291 were not genotyped