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Characterization of the sodium alginate encapsulated LS180 spheroid model as a function of time: (a) Soluble protein content per spheroid (μg protein); (b) intracellular adenosine triphosphate content per μg protein (μM/μg); (c) extracellular adenylate kinase release per μg protein (n = 3, error bars = standard deviation; statistically significant: * = p < 0.05; # = p < 0.001 (one-way ANOVA followed by the Dunnett posthoc test for comparison with time point 0)).
