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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: J Am Chem Soc. 2020 Mar 19;142(13):6128–6138. doi: 10.1021/jacs.9b13407

Figure 1.

Figure 1.

TASIN binding to EBP leads to selective toxicity in APC mutant cells. (a) Chemical structure of TASIN-2, a photoaffinity probe. (b) SDS-PAGE analysis of DLD-1 cells incubated with different TASIN-2 concentrations in the presence or absence of UV light. (c) SDS-PAGE gel of DLD-1 cell lysates treated with TASIN-2 in the presence of different concentrations of TASIN-18, an active analog. (d) Correlation amongst 17 TASIN analogs for toxicity (IC50) and p20 binding (EC50). (e) Silver-stained SDS-PAGE gel of p20 purified from cell lysates of DLD-1 cells that were cross-linked with TASIN-2 in the presence or absence of an active competitor. (f) SDS-PAGE analysis of TASIN-2 binding to p20 in wild type and EBP null cells. (g) EBP is essential in DLD-1 cells grown in low serum conditions. Cell growth for DLD-1 cells expressing independent EBP sgRNAs in media conditions that include either 10% FBS (above) or 0.2% FBS (below).