Skip to main content
. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: J Am Chem Soc. 2020 Mar 19;142(13):6128–6138. doi: 10.1021/jacs.9b13407

Figure 5.

Figure 5.

EBP inhibition kills cells by depletion of sterols and represents a transitional point in the sterol pathway of DLD-1 in terms of sterol essentiality. Compactin phenocopies TASIN selectivity suggesting different cholesterol requirements by the two cell lines. (a) Dose response of TASIN-30 with co-titration of compactin, an HMG-CoA Reductase inhibitor. Compactin sensitizes cells to TASIN. (b) Crystal Violet stain of DLD-1 cells treated with TASIN-30 in the presence or absence of zymosterol-MCD or lathosterol-MCD dosed once per day without media change. (c) Compactin is a more potent inhibitor of proliferation in DLD-1 (APC mutant) than HCT116 (APC wild type) cells. (d) Higher concentrations of LDL are required to rescue DLD1 (APC mutant) cells from compactin toxicity.