Figure 4.

MiR-7977 decreases SIRT3 and increases Ac-SOD2/SOD2 and ROS in HK-2 cells. (a) quantitative polymerase chain reaction analysis of mir-7977 in HK-2 cells treated with mir-7977 mimics. (b) Immunofluorescence staining of SIRT3 in HK-2 cells. (c) Western blot analysis of SIRT3, Ac-SOD2, and SOD2 in HK-2 cells. (d, e) Quantification of protein expression of SIRT3 and Ac-SOD2/SOD2, respectively. (f, g) Level of ROS in HK-2 cells after transfection with mir-7977 mimics (g) and quantification (f). The scale bar represents 20 μm. Data are expressed as mean ± SD.

In a: ^*p < 0.05 compared with the 50 ng/ml insulin group, ^#p < 0.05 compared with the 50 ng/ml insulin + mir-7977 mimics control group. In b–g: ^*p < 0.05 compared with the 5 ng/ml insulin group, ^#p < 0.05 compared with the 50 ng/ml insulin group, ^△p < 0.05 compared with 50 ng/ml insulin + mir-7977 mimics control group. All data are representative of three independent experiments.

Ins, insulin; NC, normal control.