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. 2020 Apr 2;9(4):69. doi: 10.3390/biology9040069

Figure 3.

Figure 3

Molecular identification of the Fusarium spp. isolates. Molecular identification of Fusarium species by PCR amplification of the small subunit ribosomal RNA gene, internal transcribed spacer (ITS), the T12 beta-tubulin gene, the translation elongation factor-1 alpha (TEF1) gene (A), the Fusarium oxysporum f. sp. cepae (FOC) secreted in xylem genes 3 (SIX3) (B1), and the Fusarium proliferatum partial calmodulin gene (CLPRO) (B2). In the last two target genes, the ITS was used as a positive control (B3). Complex band profile (DNA fingerprinting) generated using the ISSR primers (C). Primer sets and reference to the sequences used are given in Table 1. NTC—no-template negative control in which sterile double-distilled water (DDW) was added instead of the DNA template.