Table 3:
Neuropeptide Yield and PurityA
| AMP | Length (AA) | Avg. MW (Da) | Crude Yield (mg) | Crude Purity % (RTE) | Crude Loaded (mg) | Pure Yield (mg) | Recovery % | Purity % (RTE) |
|---|---|---|---|---|---|---|---|---|
| Alarin | 25 | 2894.3 | 97 | 54 (19.1) | 96 | 10 | 19 | >99% (19.0) |
| Amyloid β 1–40C | 40 | 4329.8 | 32 | 65 (31.9) | 22 | 2.9 | 20 | 96 (32.0) |
| Amyloid β 1–42C | 42 | 4514.1 | 80 | 65 (33.8) | 21 | 5.4 | 40 | 77 (34.0) |
| Bradykinin | 9 | 1060.2 | 17 | 91 (17.6) | n/a | n/a | n/a | n/a |
| Catestatin | 21 | 2326.7 | 81 | 74 (24.5) | 81 | 16 | 27 | 96 (23.7) |
| CGA-N46B | 46 | 5363.1 | 117 | 51 (34.6) | 51 | 13 | 50 | 53 (35.3) |
| α MSHD | 13 | 1623.8 | 73 | 88 (21.2) | 73 | 28 | 44 | >99% (21.2) |
| Neurotensin | 13 | 1671.9 | 45 | 90 (22.1) | 45 | 13 | 32 | 95 (22.0) |
| Neuropeptide Y | 36 | 4271.7 | 66 | 48 (35.1) | 65 | 19 | 61 | 96 (35.1) |
| Substance PD | 11 | 1348.6 | 71 | 90 (24.0) | 70 | 15 | 24 | >99% (24.1) |
| Vasoactive Intestinal PeptideB | 28 | 3326.8 | 79 | 56 (27.0) | 54 | 25 | 83 | 69 (27.6) |
| Avg. | 25.8 | 2975.5 | 69 | 70 | 58 | 15 | 40 | 88 |
A
Characterization in Supplementary Figures 19–29. All synthesized on a 3rd Generation flow synthesizer set for speed and purified by RPFC Method 2 unless otherwise specified.
B
Purified by RPFC Method 1.
C
Purified by RP-HPLC Method 1.
D
Synthesized on a 4th Generation flow synthesizer set for length.
E
RT = retention time in minutes.