Table 1:

Currently identified RAMP-interacting GPCRs, methods of interaction, identification, and FDA approved drugs^a

Official IUPHAR Receptor Name	Class	RAMP	Cell Line	Identification Method	FDA Approved Drugsb	Clinical Indication	Ref.
Chemerin receptor 1	A	RAMP2, RAMP3	HEK293T, COS-7	BRET assay, FACS	N/Ac	---	[29]
CCR1	A	RAMP1, RAMP2, RAMP3	HEK293T, COS-7	BRET assay	N/A	---	[29]
CCR2	A	RAMP2, RAMP3	HEK293T, COS-7	BRET assay	N/A	---	[29]
CCR3	A	RAMP1, RAMP2, RAMP3	HEK293T, COS-7	BRET assay	N/A	---	[29]
CCR4	A	RAMP1, RAMP2, RAMP3	HEK293T, COS-7	BRET assay	mogamuli zumab	Cutaneous T-cell Lymphoma (Mycosis Fungoides, Sézary Syndrome)	[29]
					plerixafor	non-Hodgkin lymphoma, multiple myeloma
CCR5	A	RAMP1, RAMP2, RAMP3	HEK293T, COS-7	BRET assay, FACS	maraviroc	HIV/AIDS	[29]
					ibalizuma b	HIV/AIDS
CCR6	A	RAMP1, RAMP2, RAMP3	HEK293T, COS-7	BRET assay	N/A	---	[29]
CCR7	A	RAMP2, RAMP3	HEK293T, COS-7	BRET assay, FACS	N/A	---	[29]
CCR8	A	RAMP2, RAMP3	HEK293T, COS-7	BRET assay	N/A	---	[29]
CCR9	A	RAMP1, RAMP2, RAMP3	HEK293T, COS-7	BRET assay	N/A	---	[29]
CCR10	A	RAMP1, RAMP2, RAMP3	HEK293T, COS-7	BRET assay, FACS	N/A	---	[29]
CXCR1	A	RAMP1, RAMP2, RAMP3	HEK293T, COS-7	BRET assay	N/A	---	[29]
CXCR2	A	RAMP2, RAMP3	HEK293T, COS-7	BRET assay, FACS	N/A	---	[29]
CXCR3	A	RAMP2, RAMP3	HEK293T, COS-7	BRET assay, FACS	N/A	---	[29]
		RAMP2, RAMP3	HEK293 FreeStyle	SBA immunoassay			[28]
CXCR4	A	RAMP1, RAMP3	HEK293T, COS-7	BRET assay	plerixafor	non-Hodgkin lymphoma, multiple myeloma	[29]
CXCR5	A	RAMP3	HEK293T, COS-7	BRET assay, FACS	N/A	---	[29]
CXCR6	A	RAMP3	HEK293T, COS-7	BRET assay	N/A	---	[29]
CX3CR1	A	RAMP1, RAMP2, RAMP3	HEK293T, COS-7	BRET assay, FACS	N/A	---	[29]
XCR1	A	RAMP1, RAMP2, RAMP3	HEK293T, COS-7	BRET assay, FACS	N/A	---	[29]
ACKR1	A	RAMP1, RAMP2, RAMP3	HEK293T, COS-7	BRET assay, FACS	N/A	---	[29]
ACKR2	A	RAMP1, RAMP3	HEK293T, COS-7	BRET assay, FACS	N/A	---	[29]
ACKR3	A	RAMP1, RAMP2, RAMP3	HEK293T, COS-7	BRET assay, FACS, PLA, Confocal microscopy	plerixafor	non-Hodgkin lymphoma, multiple myeloma	[29]
		RAMP2, RAMP3	HEK293 FreeStyle	SBA immunoassay			[28]
ACKR4	A	RAMP2, RAMP3	HEK293T, COS-7	BRET assay	N/A	---	[29]
ACKR5	A	RAMP3	COS-7	FACS	N/A	---	[29]
GPR4	A	RAMP1, RAMP2, RAMP3	HEK293T, HEK293 FreeStyle	SBA immunoassay, PLA	N/A	---	[28]
GPR182	A	RAMP1, RAMP2, RAMP3	HEK293T, HEK293 FreeStyle	SBA immunoassay, PLA	N/A	---	[28]
GPER/GPR30	A	RAMP3	HEK293T	BRET assay, Co-IP, Confocal microscopy	estradiol (estrogen receptor agonist; naturally occurring)	Oral contraceptives, treatment of menopausal and perimenopausal symptoms, and hypoestrogenism	[27]
CT receptor	B	RAMP1, RAMP2, RAMP3	COS-7, CHO-P	Radioligand binding, Crosslinking analysis, Confocal microscopy	pramlintide	Type I & Type II Diabetes	[23]
		RAMP1, RAMP2, RAMP3	HEK293T, HEK293 FreeStyle	SBA immunoassay			[28]
Calcitonin receptor like receptor	B	RAMP1, RAMP2, RAMP3	Xenopus oocytes, HEK293T	Radioligand binding, FACS, Crosslinking analysis	eptinezumab, fremanez umab, glacanezumab, erenumab	Chronic Migraine	[4]
		RAMP1, RAMP2, RAMP3	HEK293T, COS-7	BRET assay, FACS, PLA, Confocal microscopy			[25, 29]
		RAMP1, RAMP2, RAMP3	HEK293T, HEK293 FreeStyle	SBA immunoassay, PLA			[28]
CRF1 receptor	B	RAMP2	HEK293S, CHO-K1	ELISA	N/A	---	[8]
		RAMP2, RAMP3	HEK293 FreeStyle	SBA immunoassay	N/A	---	[28]
CRF2 receptor	B	RAMP3	HEK293 FreeStyle	SBA immunoassay	N/A	---	[28]
GHRH receptor	B	RAMP2, RAMP3	HEK293 FreeStyle	SBA immunoassay	sermorelin	Growth hormone deficiency or growth failure, prevention of HIV-induced weight loss	[28]
GIP receptor	B	RAMP1, RAMP2, RAMP3	HEK293 FreeStyle	SBA immunoassay	N/A	---	[28]
GLP-1 receptor	B	RAMP1, RAMP2, RAMP3	HEK293 FreeStyle	SBA immunoassay	exenatide, sorafenib, lixisenatide, mecaserm in rinfabate, dulaglutide, albiglutide, conivaptan, lenalidomide	Type II Diabetes	[28, 89]
GLP-2 receptor	B	RAMP1, RAMP2, RAMP3	HEK293 FreeStyle	SBA immunoassay	teduglutide	Short Bowel Syndrome	[28]
Glucagon receptor	B	RAMP2	HEK293, COS-7	Confocal microscopy, radioligand binding	glucagon recombinant, glucagon hydrochloride, oxyphenb utazone, chlordiaze poxide	Type II Diabetes	[7, 8, 25]
		RAMP1, RAMP2, RAMP3	HEK293 FreeStyle	SBA immunoassay	N/A	---	[28]
Secretin receptor	B	RAMP3	COS-7, CHO-K1	Bimolecular fluorescence complementation, BRET assay	secretin synthetic porcine, ezetimibe, pegfilgrastim	Treat High Blood Cholesterol, Lipid Abnormalities	[26]
		RAMP1, RAMP2, RAMP3	HEK293 FreeStyle	SBA immunoassay			[28]
PTH1 receptor	B	RAMP2	HEK293, COS-7	Confocal microscopy	teriparatide, abaloparatide	Osteoporosis	[25, 89]
		RAMP1, RAMP2, RAMP3	HEK293T, HEK293 FreeStyle	SBA Immunoassay, PLA			[28, 89]
PTH2 receptor	B	RAMP3	HEK293, COS-7	Confocal microscopy	teriparatide, recombin ant parathyroid hormone	Osteoporosis	[25, 89]
		RAMP1, RAMP2, RAMP3	HEK293 FreeStyle	SBA Immunoassay			[28, 89]
PAC1 receptor	B	RAMP1, RAMP2, RAMP3	HEK293 FreeStyle	SBA Immunoassay	N/A	---	[28]
VPAC1 receptor	B	RAMP1, RAMP2, RAMP3	HEK293, COS-7	Confocal microscopy	N/A	---	[25]
		RAMP2, RAMP3	HEK293 FreeStyle	SBA Immunoassay			[28]
VPAC2 receptor	B	RAMP1, RAMP2, RAMP3	HEK293S, CHO-K1	ELISA	N/A	---	[8]
		RAMP2, RAMP3	HEK293 FreeStyle	SBA Immunoassay			[28]
CaS receptor	C	RAMP1, RAMP3	COS-7, HEK293	Confocal microscopy, Co-IP	etelcalcetide	Secondary Hyperparathyroidism	[13]
ADGRF 5	Adhesion Family	RAMP1, RAMP2, RAMP3	HEK293 FreeStyle	SBA immunoassay	N/A	---	[28]

^aIUPHAR: The International Union of Basic and Clinical Pharmacology, RAMP: Receptor activity-modifying protein, BRET: Bioluminescence Resonance Energy Transfer, FACS: Fluorescenceactivated cell sorting, SBA: Suspension bead array, PLA: Proximity ligation assay, Co-IP: Coimmunoprecipitation.

^bTo determine whether each RAMP-interacting GPCR listed in the table had an associated FDA approved drug, each GPCR was cross referenced against the public resource DrugBank [90], a database which combines drug data with drug target information, and a recent review profiling trends in GPCR drug discovery through 2017.

^cN/A acronym in the Drug column stands for None Approved and is meant to denote that for the given GPCR there are no currently approved therapies directed at this GPCR in DrugBank.