Table 2.
Genotypic analysis of BnaTFL1s mutants (BnaC03.TFL1 and BnaC09.TFL1) mutagenized by sgRNA3/4 in the T1 and T2 generations
| Lines | Generations | BnaC03.TFL1 | BnaC09.TFL1 | |||
|---|---|---|---|---|---|---|
| sgRNA3 | sgRNA4 | sgRNA3 | sgRNA4 | Early flowering | ||
| K67 | T0 | Bi-allelic (d1/d5) | Chimeric (i1–1/i1–2/WT) | Bi-allelic (i1–1/i1–2) | WT | Yes |
| K67–1 | T1 | Bi-allelic (d1/d5) | Chimeric (i1–1/i1–2/WT) | Bi-allelic (i1–1/i1–2) | WT | Yes |
| K67–1-1 | T2 | Bi-allelic (d1/d5) | Chimeric (i1–1/i1–2/WT) | Homozygous (i1–1/i1–1) | WT | Yes |
| K67–1-2 | T2 | Bi-allelic (d1/d5) | Chimeric (i1–1/i1–2/WT) | Homozygous (i1–1/i1–1) | WT | Yes |
| K67–1-3 | T2 | Bi-allelic (d1/d5) | Chimeric (i1–1/i1–2/WT) | Bi-allelic (i1–1/i1–2) | WT | Yes |
| K67–1-4 | T2 | Bi-allelic (d1/d5) | Chimeric (i1–1/i1–2/WT) | Homozygous (i1–1/i1–1) | WT | Yes |
| K70 | T0 | Bi-allelic (i1–1/i1–2) | Bi-allelic (i1–1/i1–2) | WT | WT | Yes |
| K70–1 | T1 | Bi-allelic (i1–1/i1–2) | Bi-allelic (i1–1/i1–2) | WT | WT | Yes |
| K70–1-1 | T2 | Homozygous (i1–1/i1–1) | Bi-allelic (i1–1/i1–2) | WT | WT | Yes |
| K70–1-2 | T2 | Homozygous (i1–1/i1–1) | Bi-allelic (i1–1/i1–2) | WT | WT | Yes |
| K70–1-3 | T2 | Homozygous (i1–1/i1–1) | Bi-allelic (i1–1/i1–2) | WT | WT | Yes |
| K70–1-4 | T2 | Homozygous (i1–1/i1–1) | Homozygous (i1–1/i1–1) | WT | WT | Yes |
| K85 | T0 | WT | WT | Chimeric (i1–1/i1–2/d1/d5) | WT | No |
| K85–1 | T1 | WT | WT | Chimeric (i1–1/i1–2/i1–3) | WT | No |
| K85–1-1 | T2 | WT | WT | Chimeric (i1–1/i1–2/i1–3/d1) | WT | No |
| K85–1-2 | T2 | WT | WT | Chimeric (i1–1/i1–2/i1–3/d1) | WT | No |
| K85–1-3 | T2 | WT | WT | Chimeric (i1–1/i1–2/i1–3/d1) | WT | No |
| K85–1-4 | T2 | WT | WT | Chimeric (i1–1/i1–2/d1) | WT | No |
| K105 | T0 | Chimeric (i1–1/i1–2/i1–3/WT) | WT | Chimeric (i1–1/i1–2/i1–3/i1–4/WT) | WT | No |
| K105–1 | T1 | Chimeric (i1/4d/WT) | WT | Chimeric (i1–1/i1–2/i1–3/WT) | WT | No |
| K105–1-1 | T2 | Chimeric (i1–1/i1–2/i1–3/WT) | WT | Chimeric (i1–1/i1–2/i1–3/WT) | WT | No |
| K105–1-2 | T2 | Chimeric (i1–1/i1–2/i1–3/WT) | WT | Chimeric (i1–1/i1–2/i1–3/WT) | WT | No |
| K105–1-3 | T2 | Chimeric (i1–1/i1–2/i1–3/WT) | WT | Chimeric (i1–1/i1–2/i1–3/WT) | WT | No |
| K105–1-4 | T2 | Chimeric (i1–1/i1–2/i1–3/WT) | WT | Chimeric (i1–1/i1–2/WT) | WT | No |
| K112 | T0 | Chimeric (i1–1/i1–2/5d) | WT | Chimeric (i1–1/i1–2/i1–3) | WT | No |
| K112–1 | T1 | Chimeric (i1/d5/WT) | WT | Chimeric (i1–1/i1–2/i1–3/WT) | WT | No |
| K112–1-1 | T2 | Chimeric (i1–1/i1–2/i1–3/WT) | WT | Chimeric (i1–1/i1–2/i1–3/WT) | WT | No |
| K112–1-2 | T2 | Chimeric (i1–1/i1–2/WT) | WT | Chimeric (i1–1/i1–2/i1–3/WT) | WT | No |
| K112–1-3 | T2 | Chimeric (i1–1/i1–2/WT) | WT | Chimeric (i1–1/i1–2/i1–3/WT) | WT | No |
| K112–1-4 | T2 | Chimeric (i1–1/i1–2/WT) | WT | Chimeric (i1–1/i1–2/i1–3/WT) | WT | No |
d1, d4 and d5 means one, four and five nucleotides deletion, respectively; i1 one nucleotide insertion; WT wild type; i1–1, i1–2, etc. stands for different alleles