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. 2020 May 19;15(5):e0233208. doi: 10.1371/journal.pone.0233208

Fig 4. Expression of NOX1 and RAS mutation status in colon cancer cell lines and human colorectal tumor specimens.

Fig 4

(A) NOX1 expression determined for a panel of 12 colon cancer cell lines plus HEK293-NOX1 at the mRNA level by qRT-PCR. NOX1 mRNA level is given relative to β-actin. Data represent mean ± SD for at least 3 independent experiments. Bars are color-coded according to KRAS mutation status. #, the RKO cell line is KRAS WT. (B) NOX1 expression was evaluated in a panel of 27 human colorectal cell lines at the mRNA level by quantitative real time PCR and is displayed relative to β-actin. Data represent mean ± SD for at least 3 independent experiments. (C, D) Relative NOX1 mRNA expression Z score in (C) 62 cell lines of the Cancer Cell Line Encyclopedia (CCLE) and (D) 623 colorectal adenocarcinoma specimens from The Cancer Genome Atlas (TCGA) stratified according to RAS mutation status. Each dot represents a cell line or tumor sample and the coloring corresponds to the RAS mutation status. (E) Mean NOX1 mRNA expression in 51 normal colorectal tissues from TCGA colorectal cohort was compared to expression in the 623 colorectal adenocarcinoma tumor samples from the same cohort shown in (D), divided into RAS WT (n = 384) and RAS mutant (n = 239) groups. Data are ± SEM. Statistical significance for RAS WT vs. RAS mutant subcategories by the Mann-Whitney test: *p<0.05, **p<0.01, ***p<0.001; n.s. denotes the absence of statistical significance.