Table 2. RAS mutation status and NOX1 mRNA expression in 28 colon cell lines from the ATCC.
Status | ATCC cell line | RAS mutation | NOX1/β-actin × 106 | Origin | Corroboration in literature |
---|---|---|---|---|---|
SW403 | KRAS G12V | 120303 | Colorectal adenocarcinoma | [47–50] | |
SW480 | KRAS G12V | 47 | Colorectal adenocarcinoma | [47, 49–51] | |
SW620 | KRAS G12V | 3 | Colorectal adenocarcinoma | [47, 49–51] | |
LS174T | KRAS G12D | 96065 | Colorectal adenocarcinoma | [49–51] | |
LS123 | KRAS G12S | 144 | Colorectal adenocarcinoma | [49] | |
SW837 | KRAS G12C | 95 | Colorectal adenocarcinoma | [47–50] | |
DLD-1 | KRAS G13D | 8546 | Colorectal adenocarcinoma | [50, 51] | |
HCT-8 | KRAS G13D | 6748 | Colorectal adenocarcinoma | [48] | |
T84 | KRAS G13D | 1157 | Colorectal carcinoma | [47–49] | |
LoVo | KRAS G13D | 219 | Colorectal adenocarcinoma | [47–51] | |
HCT-15 | KRAS G13D | 198 | Colorectal adenocarcinoma | [47–51] | |
HCT-116 | KRAS G13D | 118 | Colorectal carcinoma | [47–51] | |
SW948 | KRAS Q61L | 1437 | Colorectal adenocarcinoma | [48–51] | |
HRAS mutant CRC | SW1116 | HRAS G161R | 494 | Colorectal adenocarcinoma | KRAS G12A [48–51] |
SW48 | HRAS G161R | 7 | Colorectal adenocarcinoma | KRAS WT [48–51] | |
RAS WT CRC | HT-29 | RAS WT | 33697 | Colorectal adenocarcinoma | [48–51] |
WiDr | RAS WT | 33513 | Colorectal adenocarcinoma | [50, 51] | |
Caco-2 | RAS WT | 16975 | Colorectal adenocarcinoma | [48, 50, 51] | |
COLO 201 | RAS WT | 10 | Colorectal adenocarcinoma | [48] | |
COLO 205 | RAS WT | 931 | Colorectal adenocarcinoma | [48–50] | |
COLO 320HSR | RAS WT | 21 | Colorectal adenocarcinoma | [49–51] | |
COLO 320DM | RAS WT | 40 | Colorectal adenocarcinoma | [48, 50, 51] | |
LS1034 | RAS WT | 45194 | Colorectal carcinoma | KRAS A146T [49–51] | |
SW1417 | RAS WT | 22 | Colorectal adenocarcinoma | [48, 49] | |
RKO | RAS WT | 4 | Colon carcinoma | [48–51] | |
RKO-E6 | RAS WT | 1 | Derived from RKO; disrupted p53 function | [48–51] | |
p53R | RAS WT | 1 | Derived from RKO | [52] | |
RAS WT normal colon | CCD-18Co | RAS WT | 85 | Normal colon fibroblast | [53] |
Mutations were identified by Sanger sequencing, and NOX1 mRNA level was measured by PCR and normalized to β-actin. CRC, colorectal cancer.