Fig 1. Effect of SNPs in random 201 nucleotide sequences on protein binding.

(A) and (C) Averages and (B) and (D) standard deviations for the change in effective RNA-protein binding free energy, ΔΔG, in response to six different single nucleotide sequence alterations averaged over 100 randomly chosen RNA sequences. (A) and (B) show data for a protein with a 7 bp footprint and (C) and (D) for a protein with a 10 bp footprint. The sequence alteration location (indicated by the dashed vertical red line) is static while the protein binding site start position is variable.