Figure 2. mHtt-Induced sLNv Loss and Aggregate Formation in Clk^Jrk/+ Flies.

(A) The number of sLNvs present per brain hemisphere is indicated for various genotypes where HttQ0 and HttQ128 indicate fragments of Htt with (Q128) and without (Q0) polyglutamines. per01 and Jrk indicate per⁰¹ and Clk^Jrk mutants (n = 17~29; *p < 0.05, **p < 0.01, ***p < 0.005).

(B) Representative images of LNvs (sLNv and lLNv) expressing HttQ25-eGFP and HttQ46-eGFP (in green) are shown for wild-type (+/+) and Clk^Jrk/+ (Jrk/+). Co-staining for PDF is indicated in red. White arrowheads indicate lLNvs. White dot circles label sLNvs without aggregates. Yellow dash circles label sLNvs with aggregates. Example aggregates are pointed out by orange arrows.

Scale bar indicates 10 μm.