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. Author manuscript; available in PMC: 2020 May 19.
Published in final edited form as: Cell Rep. 2019 Apr 2;27(1):59–70.e4. doi: 10.1016/j.celrep.2019.03.015

Figure 3. Exposure to 10 h Light, 10 h Dark Cycles Reduces mHtt-Mediated sLNv Cell Loss and Aggregate Formation.

Figure 3.

(A) The number of sLNvs present per brain hemisphere at age day 5 is indicated for various genotypes and environmental cycles, where HttQ0 and HttQ128 indicate fragments of Htt with (Q128) and without (Q0) polyglutamines. per+ and perS indicate wild-type and perS mutants (n = 36–62).

(B) Percentage of LNvs containing HttQ72-eGFP aggregates are indicated under 12 h light, 12 h dark conditions (12-12) and 10 h light, 10 h dark conditions (10-10; n = 37–39).

(C) Representative images of LNvs (sLNv and lLNv) expressing HttQ72-eGFP (in green) are shown under 12:12 and 10:10 conditions. Co-staining for PDF is indicated in red. White arrowheads indicate lLNvs. White dot circles label sLNvs without aggregates. Yellow dash circles label sLNvs with aggregates. Example aggregates are pointed out by orange arrows.

*p < 0.05, **p < 0.01, ***p < 0.005. Scale bar indicates 10 μm.