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. Author manuscript; available in PMC: 2020 May 19.
Published in final edited form as: Cell Rep. 2019 Apr 2;27(1):59–70.e4. doi: 10.1016/j.celrep.2019.03.015

Figure 6. Hop RNAi Suppresses mHtt Aggregate Formation and Increases pdfGAL4-Driven HttQ25-eGFP Levels.

Figure 6.

(A) Percentage of LNvs (labeled with PDF in red; B) at age day 7 containing HttQ72-eGFP aggregates (in green) or in a TRiP RNAi library control background (TRiP Ctrl) and expressing Hop TRiP RNAi lines (RNAi TRiP #2; n = 26–29; *p < 0.05, **p < 0.01, ***p < 0.005). White arrowheads indicate lLNvs. White dot circles label sLNvs without aggregates. Yellow dash circles label sLNvs with aggregates. Example aggregates are pointed out by orange arrows. Scale bar indicates 10 μm.

(C) Representative images of LNvs (sLNv and lLNv) expressing HttQ25-eGFP are shown in TRiP RNAi library control background (TRiP Ctrl) and expressing Hop TRiP RNAi lines (Hop RNAi TRiP #2). Asterisks indicate sLNvs.

(D) GFP intensity in the sLNvs and lLNvs for TRiP Ctrl and Hop TRiP RNAi lines is quantified and shown (n = 8–20).

Scale bars indicates 10 μm. (n = 8–20; *p < 0.05, **p < 0.01, ***p < 0.005.