KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-PDF | Developmental Studies Hybridoma Bank (DSHB) | PDF C7 |
| Rabbit polyclonal anti-PER | Rosbash Lab | N/A |
| Goat anti-Mouse IgG Alexa Fluor 488 | Invitrogen | A-11001 |
| Goat anti-Rabbit IgG Alexa Fluor 488 | Invitrogen | A-11008 |
| Donkey anti-Mouse IgG Alexa Fluor 594 | Invitrogen | A-21203 |
| Donkey anti-Rabbit IgG Alexa Fluor 594 | Invitrogen | A-21207 |
| Donkey anti-Rabbit IgG Alexa Fluor 647 | Invitrogen | A-31573 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| D(−)-APV | Sigma-Aldrich | A8054 |
| Tetrodotoxin | Sigma-Aldrich | T8024 |
| DNQX | Sigma-Aldrich | D0540 |
| Critical Commercial Assays | ||
| PicoPure RNA Isolation Kit | Applied Biosystems | KIT0204 |
| RNeasy Mini Kit | QIAGEN | 74104 |
| SuperScript III First-Strand Synthesis System | Invitrogen | 18080051 |
| RQ1 RNase-Free DNase | Promega | M6101 |
| SsoAdvanced Universal SYBR® Green Supermix | BIO-RAD | 1725274 |
| Experimental Models: Organisms/Strains | ||
| D. melanogaster: RNAi control for attP2 insertion lines: y[1] v[1]; P{y[+t7.7] = CaryP}attP2 | Bloomington Drosophila Stock Center | BDSC 36303 |
| D. melanogaster: RNAi of Hop (#1): y[1] sc[*] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00779}attP2 | Bloomington Drosophila Stock Center | BDSC 32979 |
| D. melanogaster: RNAi of Hop (#2): y[1] sc[*] v[1]; P{y[+t7.7] v[+t1.8] = TRiP.HMS00965}attP2 | Bloomington Drosophila Stock Center | BDSC 34002 |
| D. melanogaster: overexpression of HttQ0 and HttQ128: UAS-HttQ0/128 | Lee et al., 2004 | N/A |
| D. melanogaster: overexpression of eGFP tagged HttQ25, HttQ46, HttQ72 and HttQ103: UAS-HttQ25/46/72/103-eGFP | Zhang et al., 2010 | N/A |
| D. melanogaster: overexpression of Abeta42: P{UAS-Abeta1-42.G}1 | Bloomington Drosophila Stock Center | BDSC 32038 |
| D. melanogaster: overexpression of TDP43A315T: UAS-TDP43-A315T-RFP | Guo et al., 2011 | N/A |
| w[1118]; P{w[+mC] = UAS-sgg.B}MB5 | Bloomington Drosophila Stock Center | BDSC 5361 |
| D. melanogaster: Gal4 transgene expressed by Pdf promoter: Pdf-Gal4 | Renn et al., 1999 | N/A |
| Oligonucleotides | ||
| Hop forward primer: AGCGTACAACGAGGGTCTCAAG | N/A | N/A |
| Hop reverse primer: TGCCCAGTTCCTTCTCCTTCC | N/A | N/A |
| Hsp70, Hsp40 and hsf forward and reverse primers | Gonsalves et al., 2011 | N/A |
| T7 oligo dT primer: GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGGTTTTTTTTTTTTTTTTTTTTTTTT | Nagoshi et al., 2010 | N/A |
| tim forward primer: GACTTGCCAAATCCCTCATC | N/A | N/A |
| tim reverse primer: GAAGCACTGCAACTCGATCA | N/A | N/A |
| Rp49 forward primer: CCGCTTCAAGGGACAGTATCTG | N/A | N/A |
| Rp49 reverse primer: ATCTCGCCGCAGTAAACGC | N/A | N/A |
| Cam forward primer: GGCACCATCACAACAAAGG | N/A | N/A |
| Cam reverse primer: CTTCTCGGATCTCCTCTTCG | N/A | N/A |
| Software and Algorithms | ||
| DAM Data Acquisition Software | Trikinetics | http://www.trikinetics.com |
| DAM File Scan Software | Trikinetics | http://www.trikinetics.com |
| ClockLab | Actimetrics | http://www.actimetrics.com/products/clocklab/ |
| Counting Macro | Pfeiffenberger et al., 2010b | N/A |
| Boot eJTK | Hutchison et al., 2018 | https://github.com/alanlhutchison/BooteJTK |
| kallisto | Bray et al., 2016 | https://github.com/pachterlab/kallisto |
| tximport | Soneson et al., 2015 | http://bioconductor.org/packages/release/bioc/html/tximport.html |
| Nikon NIS Elements | Nikon | https://www.nikon.com/products/microscope-solutions/lineup/img_soft/nis-elements/ |