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. 2020 May 15;12:3489–3501. doi: 10.2147/CMAR.S241795

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© 2020 Wu et al.

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miR-665 is downregulated in GC tissues and cells. (A) Volcano plot of 110 differentially-expressed miRNAs. Red dots represent 91 upregulated miRNAs and green dots represent 19 downregulated miRNAs (cut-off criteria are P < 0.05 and |log2FC| > 1.0). (B) Kaplan–Meier survival analysis of correlation between miR-665 expression level and overall survival from TCGA. (C) miR-665 was significantly downregulated in four GC cell lines (AGS, HGC-27, MKN-45, and MGC-803) compared to gastric epithelial cell line (GES-1). (D) qRT-PCR analysis of miR-665 expression in 63 GC and adjacent normal tissues. Data are shown as –ΔΔCT values. Expression level data are presented as mean ± SE. *P < 0.05.