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. 2020 May 15;12:3489–3501. doi: 10.2147/CMAR.S241795

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© 2020 Wu et al.

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Restoration of CRIM1 expression abolished the effects of miR-665 on the proliferation, migration and invasion in GC cells. MGC-803 and HGC-27 cells were co-transfected with the pcDNA3.1-CRIM1 plasmid or empty vector and miR-665 mimics or negative controls. All post transfected cells were collected and used in the following experiments. (A and B) CRIM1 mRNA expression and protein expression in MGC-803 and HGC-27 cells transfected with pcDNA3.1-CRIM1 plasmid or empty vector was examined by qRT-PCR and Western blotting, respectively. (C) Transwell assays were conducted to examine post transfected cells migration and invasion, respectively. (D) Western blotting was used to detect the effects of post transfected cells on EMT-related proteins (E-cadherin, N-cadherin, and vimentin) in MGC-803 and HGC-27 cells. Data are presented as mean ± SD. *P < 0.05, **P < 0.01.