Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Real-Time RT-PCR Primers Applied in This Experiment
| Gene | Primers (5ʹ→3ʹ) | Product Size (bp) | TM (°C) |
|---|---|---|---|
| DGCR8 | F: GAAACACGACCCTCCTCTGA R: GGGTCAGCACCCATAGAGTC |
183 | 60 |
| ACTB | F: CCAACCGCGAGAAGATGA R: AGGGCATACCCCTCGTAGAT |
167 | 60 |
Abbreviations: bP, base pairs; F, forward; R, reverse; TM, melting temperature.
