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siRNA prediction of CYP51-dsRNA constructs using siFi21. Total and efficient siRNAs of 21 nt were predicted with the siFi21 software for the cDNA of the respective FgCYP51 target genes FgCYP51A, FgCYP51B and FgCYP51C.
| Construct | Total siRNA hits | Efficient siRNA hits |
|---|---|---|
| CYPA-500 | 480 | 240 |
| CYPA-800 | 780 | 390 |
| CYPA-full | 1498 | 740 |
| CYPB-400 | 380 | 183 |
| CYPB-800 | 780 | 387 |
| CYPB-full | 1555 | 768 |
| CYPC-400 | 380 | 186 |
| CYPC-800 | 780 | 385 |
| CYPC-full | 1528 | 759 |
