Figure 6.

The EV-A71 3D/3CD in infected cell associates with ribosome complex despite polysome disruption. (A) Polysome profiling of RD cells infected with EV-A71 MP4 strain at MOI of 10 was analysed as described in Fig. 6. MP4 infection (marked in red) disrupted the polysome peaks while the 80S monosome peak increased when compared with the profile of mock-infected cell (black). (B) Cleavage of eIF4G upon EV-A71 infection was examined by immunoblotting using anti-eIF4G antibody. Expression of EV-A71 3D and 3CD proteins was analysed by using anti-EV-A71 3D antibody. Proteins level of RPS6, RPL0, and β-actin served as the loading control. (C) Immunoblotting was performed to analyse the distributions of EV-A71 3D/3CD and 3A/3AB in the sucrose gradient using antibody against EV-A71 3D and 3A, respectively. Fractions representative of different ribosomal assemblies were verified by antibodies against RPS6 and RPL0. In. represented 5% lysates layered onto the gradient in the beginning.