Figure 8.

The 3D protein directly activates both cap- and IRES-dependent translation in vitro. In vitro translation was performed in 60% S10 extracts prepared from HEK293T cells in the presence of the recombinant EV-A71 3D proteins (lane 3–5) or not (lane 1–2) to analyse (A) cap- and (B) IRES-dependent translation. Ctrl: control group, +Cap: lysates pretreated with 1mM m⁷G cap analogue. The recombinant EV-A71 3D proteins in each reaction were examined by immunoblotting using anti-EV-A71 3D antibody. Ribosomal proteins RPS6 and RPL0 served as the loading controls. Statistical analysis was performed by the two-way ANOVA. ***: p <0.001; ns: not significant.