Figure 7.

Mitochondrial translation following the loss of TRMT2B.

(A) Western blot analysis of steady-state levels of OXPHOS subunits in HAP1 parental cell line (WT), and the HAP1 TRMT2B knockout cell line (KO). (B) Assessment of de novo mitochondrial protein synthesis through [35S]-methionine incorporation in the HAP1 WT and KO cell lines. Coomassie gel staining was used as a control for protein loading. (C) Growth curves obtained by Incucyte kinetic imaging system of HAP1 WT and KO cell lines. Cells were grown for 140 hours in the presence of ‘high’ (4.5 g/L) or ‘low’ (1 g/L) glucose.