Figure 2.
TRMT2B forms methylated m5U54 residue of mouse mitochondrial tRNAIle. (A) Secondary structure of mouse mitochondrial tRNAIle [41]. Inset the closeup of the T-loop consensus of mouse mitochondrial tRNA species containing the methylated residue m5U54. (B) Mass spectra of mouse mitochondrial tRNAIle fragment with U54 nucleotide digested by RNase T1. The upper panel corresponds to wild type cells, the middle panel corresponds to Trmt2B knockout cells, while the lower panel corresponds to the complementation of Trmt2B knockout cells bya reintroduced Trmt2B gene. Mass of 1953 Da corresponds to a fully methylated fragment, 1939 Da – a fragment without a methyl group.