Figure 4.

Influence of CRM1 and/or NXF1 inhibition on FL RNA packaging. (A) Schematic maps of the MLV clones expressed into the NIH3T3 cells. The insertion of GFP in gag gene, is represented in grey and enables the production of Gag in cis in native level. The NV-MS2 construct producing a non-viral RNA was used as negative control as described in [25]. The visualization of MS2-tagged RNA was based on the interaction with the MS2 coat protein fused to mCherry (dark grey) and co-transfected with the MLV plasmids. (B) The graph represents the packaging ability of MLV in each condition. The number of grey dots colocalized with dark grey dots was determined and packaging efficiencies were calculated as the ratios of Gag with RNA: total Gag. n ≥ 700 virions. Percentages are presented as mean ± SEM.