Figure 4.

Determination of the RNA and protein content in SEC fractions.

Using our newly established methods, miRNAs were quantified in 25 SEC fractions derived from 500 µl plasma or serum. In both, plasma and serum, miR-16 and miR-142 extracted and amplified by our methods were found in the early fractions, whereas proteins measured by the Bradford assay were detected in the late fractions (A). SDS-PAGE of total proteins in 25 SEC fractions of plasma or serum. The samples on all gels are derived from the same experiment and all gels were processed in parallel. (B).