Figure 4.

Knockdown of FTSJ3 inhibits cell proliferation and survival in breast cancer cell lines. (A) Expression levels of FTSJ3, measured by qRT-PCR and western blot assays, in a panel of 13 breast cancer cell lines plus MCF10A. mRNA expression levels in the immortalized but nontumorigenic breast epithelial cell line MCF10A cells were arbitrarily set as 1. Three stars indicate the cell lines with FTSJ3 gene amplification. (B) Knockdowns of FTSJ3 in SUM52 and MCF7 cells with two different siRNAs were confirmed by qRT-PCR and western blot assays. (FJ3 = FTSJ3). (C) Bar graph shows relative cell growth after knocking down FTSJ3 in SUM52 and MCF7 breast cancer cells (** p < 0.01, *** p < 0.001). Data are expressed as mean ± SD. (D) FTSJ3 depletion results in cell death 2 days after siRNA transfection in SUM52 cells, measured by flow cytometry and double staining with Annexin V-FITC and PI. These experiments were repeated independently three times with similar results.