Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Sptb | https://www.ncbi.nlm.nih.gov/gene/20741 | Gene ID: 20741 | |
| Gene (M. musculus) | Sptbn4 | https://www.ncbi.nlm.nih.gov/gene/80297 | Gene ID: 80297 | |
| Genetic reagent (M. musculus) | Nestin-cre | The Jackson Laboratory |
Stock No:003771 | |
| Genetic reagent (M. musculus) | Sptbflox/flox | PMID:32052742 | ||
| Genetic reagent (M. musculus) | Sptbn4flox/flox | PMID:30226828 | ||
| Antibody | Anti-Ankyrin G (Mouse monoclonal) | Neuromab | Clone: N106/36; RRID:AB_10673030 | IF (1:500) |
| Antibody | Anti-Ankyrin G (Mouse monoclonal) |
Neuromab | Clone N106/65; RRID:AB_10675130 | IF (1:100) for STED |
| Antibody | Anti-Ankyrin R (Mouse monoclonal) | Neuromab | Clone N380/A10; RRID:AB_2491109 | IF (1:500) |
| Antibody | Anti-Parvalbumin (Mouse monoclonal) | Neuromab | Clone L114/3; RRID:AB_2651167 | IF (1:500) |
| Antibody | Anti-PanNav (Mouse monoclonal) | Neuromab | Clone: N419/78; RRID:AB_2493099 | IF (1:300) |
| Antibody | Anti-PanNav (Mouse monoclonal) | Sigma-Aldrich | Clone: K58/35; RRID:AB_477552 | IF (1:300) |
| Antibody | Anti-β1 spectrin (Mouse monoclonal) | Neuromab | Clone: N385/21; RRID:AB_2315815 | IF (1:500) |
| Antibody | Anti-β4 spectrin SD antibody (Rabbit polyclonal) | PMID:28123356 | IF (1:500) WB (1:1000) |
|
| Antibody | Anti-Ankyrin R (Rabbit polyclonal) | PMID:25362473 | IF (1:500) | |
| Antibody | Anti-Caspr (Rabbit polyclonal) | PMID:10460258 | RRID:AB_2572297 | IF (1:500) |
| Antibody | Anti-Pan Neurofascin (Chicken polyclonal) | R and D Systems | Cat.#: AF3235; RRID:AB_10890736 | IF (1:500) |
| Antibody | Anti-MAP2 (Chicken polyclonal) | Encor | Cat.#: CPCA-MAP2; RRID:AB_2138173 | IF (1:500) |
| Antibody | Anti-GFP (Rat monoclonal) | Biolegend | Cat.#: 338002; RRID:AB_1279414 | IF (1:500) |
| Antibody | Anti-Parvalbumin (Rabbit polyclonal) | Novus | RRID:AB_791498 | IF (1:500) |
| Antibody | Anti-active Caspase 3 (Rabbit polyclonal) |
R and D Systems | RRID:AB_2243952 | IF (1:500) |
| Antibody | Anti-βAPP (Rabbit polyclonal) | Thermo Fisher Scientific | RRID:AB_2533902 | IF (1:1000) |
| Sequence-based reagent | Genotyping primer for Sptbn4flox/flox mouse (sense) | PMID:30226828 | 5’-GAGCTGCATAAGTTCTTCAGCGATGC-3’ | |
| Sequence-based reagent | Genotyping primer for Sptbn4flox/flox mouse (anti-sense) | PMID:30226828 | 5’-ACCCCATCTCAACTGGCTTTCTTGG-3’ | |
| Sequence-based reagent | Genotyping primer for Sptbflox/flox mouse (sense) | PMID:32052742 | 5’- ACAGAGACAGATGGCCGAAC-3‘ | |
| Sequence-based reagent | Genotyping primer for Sptbflox/flox mouse (anti-sense) | PMID:32052742 | 5’-CTCTGGTTCCCAGGAGAGC-3’ | |
| Sequence-based reagent | Genotyping primer for Avil-cre mouse (primer 1) | PMID:29038243 | 5’-CCCTGTTCACTGTGAGTAGG-3’ | |
| Sequence-based reagent | Genotyping primer for Avil-cre mouse (primer 2) | PMID:29038243 | 5’- AGTATCTGGTAGGTGCTTCCAG-3’ | |
| Sequence-based reagent | Genotyping primer for Avil-cre mouse (primer 3) | PMID:29038243 | 5’-GCGATCCCTGAACATGTCCATC-3’ | |
| Transfected construct (Rat) | pEGFP-N1-AnkG-270kDa | PMID:9744885 | Transfected construct (Rat) | |
| Transfected construct (Human) | pEGFP-N1-AnkR | This paper | Transfected construct (Human) Rasband laboratory |
|
| Transfected construct (Human/Rat) | pEGFP-N1- AnkR/G chimera |
This paper | Transfected construct (Human/Rat) Rasband laboratory |
|
| Sequence-based reagent | AnkR-F | This paper | 5’-ATCTCGAGATGCCCTATTCTGTGG-3’ Rasband laboratory |
|
| Sequence-based reagent | AnkR-R | This paper | 5’-AGCTTGAGGGGGTTGGGTGTCGA-3’ Rasband laboratory |
|
| Sequence-based reagent | pEGFP-N1-F | This paper | 5’-CCAACCCCCTCAAGCTTCGAATTCTG-3’ Rasband laboratory |
|
| Sequence-based reagent | pEGFP-N1-R | This paper | 5’-TAGGGCATCTCGAGATCTGAGTCC-3’ Rasband laboratory |
|
| Sequence-based reagent | AnkR-SBD-F | This paper | 5’-CCCCTGGTACAGGCAACGTTCCCGGAGAATG-3’ Rasband laboratory |
|
| Sequence-based reagent | AnkR-SBD-R | This paper | 5’-ACTGTTTTGTATCGCAGGGCCAG-3’ Rasband laboratory |
|
| Sequence-based reagent | AnkG-RD-F | This paper | 5’-TGCGATACAAAACAGTTGAACGGAG-3’ Rasband laboratory |
|
| Sequence-based reagent | AnkG-RD-R | This paper | 5’- GTACCGTCGACTGCAGAATTCGGTGGGCTTTCTTCTC-3’ Rasband laboratory |
|
| Sequence-based reagent | AnkR-RD-F | This paper | 5’- TCCGATATCAGCATTCTCAGTGAGTCC-3’ Rasband laboratory |
|
| Sequence-based reagent | AnkR-RD-R | This paper | 5’- TAGAATTCGGGGGTTGGGTGTCGAGGTG-3’ Rasband laboratory |
|
| Commercial assay or kit | GeneArt Seamless Cloning and Assembly Kit |
Thermo Fisher Scientific | Cat#: A13288 | |
| Software, algorithm | Zen | Carl Zeiss | RRID:SCR_013672 | |
| Software, algorithm | Labchart 8.0 | ADI Systems | RRID:SCR_017551 | |
| Software, algorithm | Leica Application Suite X | Leica | RRID:SCR_013673 | |
| Software, algorithm | Fiji | National Institutes of Health | RRID:SCR_002285 | |
| Software, algorithm | Prism | Graph Pad | RRID:SCR_002798 | Version 6 |