Figure 1. mpeg1+ primitive macrophages on the yolk in control and csf1r^DM larvae.

(A) Representative images of mpeg1+ macrophages located on the yolk (29 hpf) and quantification of mpeg1+ cell numbers over time. (B) Representative images of mpeg1+ positive primitive macrophages at 52 hpf. The dotted line indicates the border between the yolk (I) and the embryonic tissue (II). Quantification of mpeg1+ macrophages that colonized the tissue (II) and primitive macrophages located on the yolk (I). (C) Representative maximum projection of long term time lapse imaging of control and csf1r^DM larvae showing migratory trajectories of mpeg1+ macrophages. (D) Snap shots from dividing mpeg1+ primitive macrophages in control and csf1r^DM larvae (~36 hpf) and quantification of proliferative primitive macrophages during 16 hr time lapse imaging (~32 hpf – 48 hpf) (control n = 5, csf1r^DM n = 3). (E) Quantification of fraction proliferative embryonic macrophages during 16 hr time lapse imaging (~56 hpf – 72 hpf) in control and csf1r^DM larvae (n = 3 per group). Scale bars represent 100 µM. Error bars represent standard deviation. Statistical significance is calculated using one-way ANOVA with Dunnett’s multiple comparison test or Student’s t-tests *<0.05 **<0.01 ***<0.001. mpeg1+ cells were quantified on one side of the embryo (right side). Each dot represents one fish.

Figure 1—figure supplement 1. Mpeg1+ cells can be detected in the tail region of control and csf1r^DM larvae.

(A) Representative images of mpeg1+ myeloid progenitors located in the tail region at 32 hpf and quantification. (B) Representative images of mpeg1+ myeloid progenitors located in the tail region at 50 hpf and quantification. Scale bars represent 100 µm. Error bars represent standard deviation. Statistical significance is calculated using Student’s t-tests ***<0001. Mpeg1+ cells were quantified on one side of the embryo (right side). Each dot represents one fish.