Figure 4. Two morphologically distinct populations of mpeg1+ cells in emerge from 15 dpf in the zebrafish skin.

(A) Representative images of a control and csf1r^DM zebrafish at 20 dpf. Dotted line represents the outline of the fish and its eye. (B) Quantification of the total number of mpeg1+ cells at one unilateral side of the fish at different time points between 8 and 24 dpf. The number of mpeg1+ cells was manually counted from the unilateral side presented in panel A. Plot showing the relationship between number of mpeg1+ cells and fish size. Each dot represents one fish. (C) Representative images of mpeg1+ cells in different body regions at 22 dpf showing differences in morphology between controls and csf1r^DM or il34^-/-mpeg1+ cells (n = 3 per group). Error bars represent standard deviation. Mpeg1+ cells were quantified on one side of the embryo (right side).

Figure 4—figure supplement 1. Abnormal morphology of csf1r^DM and il34^-/-, but not csf1a^-/-b^-/- larval mpeg1+ cells in the skin.

(A) Representative images of a control, csf1r^DM and il34^-/- fish at 22 dpf. Dotted line represents the outline of the fish and its eye. (B) Representative images of mpeg1+ cells in the skin of csf1a^-/-b^-/- showing no differences in morphology between control and csf1a^-/-b^-/-mpeg1+ cells at 15 dpf (n = 3 per group). Error bars represent standard deviation.