| Gene | Species | Forward primer (5’−3’) | Reverse primer (5’−3’) |
|---|---|---|---|
| hsp70 (C12C8.1) | C. elegans | TTGGTTGGGGGATCAACTCG | GAGCAGTTGAGGTCCTTCCC |
| hsp70 (F44E5.4/.5) | C. elegans | CTATCAGAATGGAAAGGTTGAG | TCTTTCCGTATCTGTGAATGCC |
| pmp-3 | C. elegans | TAGAGTCAAGGGTCGCAGTG | ATCGGCACCAAGGAAACTGG |
| syp-1 | C. elegans | GATGAAATGATAATTCGCCAAGA | ACGCAATCTTCCCTCATTTG |
| act-1 | C. elegans | ATCACCGCTCTTGCCCCATC | GGCCGGACTCGTCGTATTCTTG |
| gpd-3 | C. elegans | CAATGCTTCCTGCACCACTA | CTCCAGAGCTTTCCTGATGG |
| Hsp27 (Hspb1) | Mouse | ATCCCCTGAGGGCACACTTA | GGAATGGTGATCTCCGCTGAC |
| Hsp70 (Hspa1a) | Mouse | ATGGACAAGGCGCAGATCC | CTCCGACTTGTCCCCCAT |
| Cryab (Hspb5) | Mouse | CGGACTCTCAGAGATGCGTT | TGGGATCCGGTACTTCCTGT |
| Gapdh | Mouse | AACGACCCCTTCATTGAC | TCCACGACATACTCAGCAC |
| HSP70 (HSPA1A) | Human | CTACAAGGGGGAGACCAAGG | TTCACCAGCCTGTTGTCAAA |
| SPT16 (SUPT16H) | Human | GTGGAAAAGGCCATTGAAGA | GTGATAGCCCCAAAGTGCAT |
| GAPDH | Human | GAAGGTGAAGGTCGGAGTC | GAAGATGGTGATGGGATTTC |
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