Gene name	Position	Forward primer (5’−3’)	Reverse primer (5’−3’)	Amplicon size	Antibody used
C12C8.1	Promoter	CTCAGGCAGTGGAAGAACTAAA	TTATACGTTCCTCTGGCATCTTC	88 bp	FLAG (HSF1-FLAG), H3, HA (HMG-3-HA)
F44E5.4/.5	Promoter	ATACTACCCGAATCCCAGCC	GCAACAGAGACGCAGATTGT	150 bp	FLAG (HSF1-FLAG), H3, HA (HMG-3-HA), RNA polymerase II
C12C8.1	Region A	ATCGACTTGGGTACTACGTACTC	CTTGTTCCCTTCGGAGTTCG	161 bp	RNA polymerase II
syp-1	Promoter	CAACAAAACGCGCTCCATT	GGAGGCCGCAAACACC	80 bp	FLAG (HSF1-FLAG)
C12C8.1	Region B	TGTACTTGGGCATTCTGTACGG	GCATTGAGTCCAGCAATAGTAGC	108 bp	RNA polymerase II, H3
C12C8.1	Region C	ACAATTCGCAATGAGAAGGGACG	GCATCTTCTGCTGATAACAGTGATC	191 bp	RNA polymerase II
F44E5.4/.5	Region B	TGATCTTCGATCTCGGAGGAGG	TCACAAGCAGTTCGGAGACG	220 bp	RNA polymerase II, H3
F44E5.4/.5	Region C	TTGATGAAACACTTCGTTGGTTGG	TCCAGCAGTTCCAGGATTTC	170 bp	RNA polymerase II
C12C8.1	TSS	ACGTACTCATGTGTCGGTAT	TCTTCTTCCAGTTTACATAATCCT	92 bp	H3, HA (HMG-3-HA)
F44E5.4/.5	TSS	TAAAAGGGCTGGGATTCGGG	ACCGAGGTCGATACCAATAGC	118 bp	H3, HA (HMG-3-HA)
C12C8.1	TSS-50	AACTCAAATCTTATGCAGAAT	CGTAGTACCCAAGTCGATTCCA	119 bp	HA (HMG-3-HA)
F44E5.4/.5	TSS-50	GTCGGCCGTCTCTTTCTCTT	CCCGAATCCCAGCCCTTTT	157 bp	HA (HMG-3-HA)