Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | Wild type C57BL/6 | Envigo | Cortical neuron preparations were made from P0 pups from timed-pregnant C57BL/6 mice | |
| Genetic reagent (C. elegans) | N2 Wild type | Caenorhabditis Genetic Center | N2, C. elegans var Bristol | |
| Genetic reagent (C. elegans) | hsf-1::3X flag I | Prahlad lab, University of Iowa | CRISPR insertion of 3X FLAG at C’ of hsf-1 | This paper (Materials and methods) |
| Genetic reagent (C. elegans) | tph-1 (mg280) II | Caenorhabditis Genetic Center | Strain Name: MT15434 | |
| Genetic reagent (C. elegans) | hsf-1(sy441)I | Caenorhabditis Genetic Center | Strain Name: PS3551 | |
| Genetic reagent (C. elegans) | lite-1(ce314); ljIs102 [tph-1;;ChR2::YFP;unc-122::gfp] | Caenorhabditis Genetic Center | Strain Name: AQ2050 | |
| Genetic reagent (C. elegans) | hmg-3(bar24[hmg-3::3xHA]) I | Gift from Dr. Baris Tursun, Max Delbrück Center (MDC) | Strain Name: BAT1560 | |
| Genetic reagent (C. elegans) | glp-4(bn2) I | Caenorhabditis Genetic Center | Strain Name: SS104 | |
| Genetic reagent (C. elegans) | rrf-1(pk1417) I | Caenorhabditis Genetic Center | Strain Name: NL2098 | |
| Genetic reagent (C. elegans) | mkcSi13 II; rde-1(mkc36) V | Caenorhabditis Genetic Center | Strain Name: DCL569 | |
| Genetic reagent (C. elegans) | rde-1(ne219) V; kbIs7 | Caenorhabditis Genetic Center | Strain Name: VP303 | |
| Genetic reagent (C. elegans) | tph-1 (mg280) II; hsf-1::flag I | Prahlad lab, University of Iowa | Cross made with hsf-1::3Xflag (I) strain and tph-1 (II) | This paper (Materials and methods) |
| Genetic reagent (C. elegans) | tph-1 (mg280) II; hmg-3(bar24[hmg-3::3xHA]) I | Prahlad lab, University of Iowa | Cross made with FLAG tagged train and tph-1 | This paper (Materials and methods) |
| Cell line (Homo-sapiens) | NTERA-2 cl.D1 (NT2 cell line) | Originally from Stratagene | A gift from Dr. Christopher Stipp, University of Iowa (PMID:10806098) | |
| Antibody | Mouse monoclonal anti-FLAG | Sigma | Catalog No. F1804 | IF (1:100), WB (1:1000) |
| Antibody | Mouse monoclonal anti-RNA polymerase II | BioLegend | Catalog No. 664906 | ChIP (5 µl per sample) |
| Antibody | Rabbit polyclonal anti-Histone H3 | Abcam | Catalog No. ab1791 | ChIP (2 µl per sample) |
| Antibody | Rabbit polyclonal anti-HA | Abcam | Catalog No. ab9110 | ChIP (5 µl per sample), WB (1:1000) |
| Antibody | Rabbit polyclonal anti-HSF1 | Cell Signaling Technology | Catalog No. 4356 | IF (1:100), WB (1:1000) |
| Antibody | Rabbit monoclonal anti-HSF1(S320) | Abcam | Catalog No. ab76183 | WB (1:1000) |
| Antibody | Mouse monoclonal anti-Hsp70 | Gift from Dr. Richard Morimoto, Northwestern University | Clone: 3A3 | WB (1:1000) |
| Antibody | Mouse monoclonal anti-α-tubulin | DSHB, University of Iowa | Catalog No. AA4.3 | WB (1:1000) |
| Antibody | Donkey anti-mouse Cy3 | Jackson ImmunoResearch Laboratories | Catalog No. 715-165-150 | IF (1:1000) |
| Antibody | AlexaFluor 488-conjugated goat anti-rabbit IgG | Invitrogen | Catalog No. A-11008 | IF (1:500) |
| Antibody | Sheep anti-mouse IgG | Rockland Immunochemicals | Catalog No. 610-631-002 |
WB (1:10,000) |
| Antibody | Goat anti-rabbit IgG | Invitrogen | Catalog No. A21109 |
WB (1:10,000) |
| Sequence-based reagent | siRNA targeting human HSF1 | Santa Cruz Biotechnology | Catalog No. sc-35611 | These are pools of 3–5 target-specific 19–25 nucleotide sequences designed by the company |
| Sequenced-based reagent | siRNA targeting human SUPT16H | Santa Cruz Biotechnology | Catalog No sc-37875 | These are pools of 3–5 target-specific 19–25 nucleotide sequences designed by the company |
| Sequenced-based reagent | Control siRNA | Santa Cruz Biotechnology | Catalog No sc-37007 | These are pools of 3–5 target-specific 19–25 nucleotide sequences designed by the company |
| Commercial assay or kit | Mouse anti-FLAG M2 magnetic bead | Sigma | Catalog No. M-8823 | ChIP (15 µl per sample) |
| Commercial assay or kit | Protein A/G magnetic bead | Pierce | Catalog No. 88802, Pierce | ChIP (15 µl per sample) |
| Commercial assay or kit | iScript cDNA Synthesis Kit | Bio-Rad | Catalog No. 170–8891 | |
| Commercial assay or kit | SYBR Green Master Mix | Roche | Catalog No. 04887352001 | |
| Commercial assay or kit | Lipofectamine LTX Plus reagent | Thermo Fisher Scientific | Catalog No. 15338030 | |
| Commercial assay or kit | ChIP DNA purification kit | Zymo Research | Catalog No. D5205 | |
| Chemical compound, drug | 5-HT | Sigma | Catalog No. 85036 | |
| Chemical compound, drug | Sumatriptan succinate (5-HT1 receptor agonist) | Sigma | Catalog No. S1198 |
|
| Chemical compound, drug | DOI hydrochloride (5-HT2A receptor agonist) | Sigma | Catalog No. D101 | |
| Chemical compound, drug | BIMU8 hydrate (5-HT4 receptor agonist) | Sigma | Catalog No. B4063 |
|
| Chemical compound, drug | ST1936 (5-HT6 receptor agonist) | Sigma | Catalog No. SML0260 |
|
| Chemical compound, drug | PKA inhibitor H89 | Sigma | Catalog No. B1427 |
|
| Software, algorithm | ImageJ (FIJI) |
NIH | Quantification of fluorescence intensity | |
| Software, algorithm | LAS X | Leica Microsystems | Confocal imaging and analysis |