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. 2020 May 19;9:e54935. doi: 10.7554/eLife.54935

Figure 2. Quantification of senescence markers in gut and testis of older tert-/- zebrafish.

Figure 2.

(A-B) Western blot analysis of DNA damage and senescence-associated proteins in gut and testis of 3 month (A) or 9-month-old (B) of WT and tert-/- siblings (N >= 5 fish). Representative western blot (left panel) and corresponding quantification (right panel) showing induction of DNA Damage Response (H2A.X-P and p53) in 3-month-old and senescence (p15/16) in 9-month-old tert-/- zebrafish. (C) RT-qPCR analysis of senescence associated genes p15/16 and p21. RT-qPCR graphs are representing mean ± SEM mRNA fold increase after normalisation by rpl13a gene expression levels (* p-value<0.05; ** p-value<0.01, using the Mann-Whitney test).

Figure 2—source data 1. Western Blot quantifications, as plotted in Figure 2A-B.
Figure 2—source data 2. Real-time qPCR data of p15/16 and p21, as plotted in Figure 2C.