Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Danio rerio) | tert hu3430/+ | Hubrecht Institute, Utrecht, Netherland | RRID:ZFIN_ZDB-GENO-100412-50 | |
| Genetic reagent (Danio rerio) | tp53 M214K/+ | Berghmans et al., 2005 | RRID:ZDB-ALT-050428-2 | |
| Genetic reagent (Danio rerio) | ztor xu015Gt/+ | Ding et al., 2011 | RRID:ZDB-ALT-120412-1 | |
| Gene (Danio rerio) | tert | ZDB-GENE-080405–1 | ||
| Gene (Danio rerio) | tp53 | ZDB-GENE-990415–270 | ||
| Gene (Danio rerio) | ztor | ZDB-GENE-030131–2974 | ||
| Gene (Danio rerio) | cdkn2a/b (p15/16) | ZDB-GENE-081104–306 | ||
| Gene (Danio rerio) | cdkn1a (p21) | ZDB-GENE-070705–7 | ||
| Gene (Danio rerio) | bcl2l1 (Bcl-XL) | ZDB-GENE-010730–1 | ||
| Gene (Danio rerio) | ppargc1a (PGC1a) | ZDB-GENE-080505–1 | ||
| Gene (Danio rerio) | rpl13a | ZDB-GENE-030131–168 | ||
| Antibody | anti-p16 (mouse monoclonal; F-12) | Santa Cruz Biotechnology | #Sc-1661; RRID:AB_628067 | IF(1:50), WB (1:700) |
| Antibody | anti-FoxO1 (rabbit monoclonal; C29H4) | Cell Signaling Technology | #2880; RRID:AB_2106495 | IF(1:50) |
| Antibody | anti-zebrafish p53 (rabbit polyclonal) | Anaspec | #55342; RRID:AB_2287635 | WB (1:1000) |
| Antibody | anti-zebrafish γH2AX (rabbit polyclonal) | GeneTex | #GTX127342; RRID:AB_2833105 | WB (1:1000) |
| Antibody | anti-SOD2 (rabbit polyclonal) | Sigma-Aldrich | #SAB2701618; RRID:AB_2833106 | WB (1:1000) |
| Antibody | anti-phospho-Akt, Ser473 (rabbit monoclonal; D9E) | Cell Signaling Technology | #4060; RRID:AB_2315049 | WB (1:1000) |
| Antibody | anti-total-Akt (rabbit polyclonal) | Cell Signaling Technology | #9272, RRID:AB_329827 | WB (1:1000) |
| Antibody | anti-phospho-FoxO1, Ser256 (rabbit polyclonal) | Cell Signaling Technology | #9461; RRID:AB_329831 | WB (1:100) |
| Antibody | anti-tubulin (mouse monoclonal; B-5-1-2) | Sigma | #T6074; RRID:AB_477582 | WB (1:5000) |
| Antibody | Alexa Fluor 568 goat anti-mouse (goat polyclonal) | Invitrogen | #A11004; RRID:AB_2534072 | IF (1:500) |
| Antibody | Alexa Fluor 488 goat anti-rabbit (goat polyclonal) | Invitrogen | #A11008; RRID:AB_143165 | IF (1:500) |
| Antibody | HRP- anti-rabbit (goat polyclonal) | Santa Cruz | #Sc2054; RRID:AB_631748 | WB (1:5000) |
| Antibody | HRP- anti-mouse (goat polyclonal) | Santa Cruz | #Sc2005; RRID:AB_631736 | WB (1:5000) |
| Sequence-based reagent | cdkn2a/b (p15/16) Fw | This paper | PCR primers | GAGGATGAACTGACCACAGCA |
| Sequence-based reagent | cdkn2a/b (p15/16) Rv | This paper | PCR primers | CAAGAGCCAAAGGTGCGTTAC |
| Sequence-based reagent | bcl2l1 (Bcl-XL) Fw | This paper | PCR primers | GGGCTTGTTTGCTTGGTTGA |
| Sequence-based reagent | bcl2l1 (Bcl-XL) Rv | This paper | PCR primers | AGAACACAGTGCACACCCTT |
| Sequence-based reagent | cdkn1a (p21) Fw | This paper | PCR primers | CAGCGGGTTTACAGTTTCAGC |
| Sequence-based reagent | cdkn1a (p21) Rv | This paper | PCR primers | TGAACGTAGGATCCGCTTGT |
| Sequence-based reagent | ppargc1a (PGC1a) Fw | This paper | PCR primers | CTGTGGAACCCCAGGTCTGAC |
| Sequence-based reagent | ppargc1a (PGC1a) Rv | This paper | PCR primers | ACTCAGCCTGGGCCTTTTGCT |
| Sequence-based reagent | rpl13a Fw | Henriques et al., 2013 | PCR primers | TCTGGAGGACTGTAAGAGGTATG |
| Sequence-based reagent | rpl13a Rv | Henriques et al., 2013 | PCR primers | AGACGCACAATCTTGAGAGCAG |
| Sequence-based reagent | cdkn2a/b (p15/16) Morpholino | This paper | morpholino | TCAGTTCATCCTCGACGTTCATCAT |
| Sequence-based reagent | Control Morpholino | GeneTools | morpholino | CCTCTTACCTCAGTTACAATTTATA |
| Commercial assay or kit | In Situ Cell Death Detection Kit, Fluorescein | Roche | 11684795910 | |
| Commercial assay or kit | CellTiter-Glo Luminescent Cell Viability Assay | Promega | G7570 | |
| Chemical compound, drug | 2′,7′-Dichlorofluorescin diacetate (DCFDA) | Sigma Aldrich | D6883 | |
| Chemical compound, drug | AKT1/2 kinase inhibitor | Santa Cruz | sc-300173 | |
| Chemical compound, drug | N-Acetyl-L-Cysteine (NAC) | Sigma Aldrich | A7250 | |
| Chemical compound, drug | MitoTEMPO | Sigma Aldrich | SML0737 | |
| Other | DAPI stain | Sigma | D9542 | (0.5 µg/mL) |