Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
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|---|---|---|---|---|---|---|---|
| Gene (D. melanogaster) | CG43236 | NA | FLYB: FBgn0262881 | NA | |||
| Gene (D. melanogaster) | E(spl)m3-HLH | NA | FLYB: FBgn0002609 | NA | |||
| Gene (D. melanogaster) | Drip | NA | FLYB: FBgn0015872 | NA | |||
| Gene (D. melanogaster) | bnl | NA | FLYB: FBgn0014135 | NA | |||
| Gene (D. melanogaster) | btl | NA | FLYB: FBgn0285896 | NA | |||
| Strain, strain background (D. melanogaster) | Oregon R | BloomingtonDrosophilaStock Center | BDSC: 5 | NA | |||
| Strain, strain background (L. boulardi) |
Leptopilina boulardi | BloomingtonDrosophilaStock Center | PMID:17967061 | Strain G486 | |||
| Genetic reagent (D. melanogaster) | HmlΔ-GAL4; UAS-2XEGFP | BloomingtonDrosophilaStock Center | BDSC: 30140; FLYB: FBst0030140 | FLYB genotype: w1118; P{Hml-GAL4.Δ}2, P{UAS-2xEGFP}AH2 | |||
| Genetic reagent (D. melanogaster) | Hml-GAL4-Lineage Trace (HLT)-GAL4 | Dr. Utpal Banerjee | PMID:22134547 |
Hml-Gal4 UAS-FLP,
ubi-FRT-STOP-FRT-Gal4 |
|||
| Genetic reagent (D. melanogaster) | lz-GAL4; UAS-GFP | BloomingtonDrosophilaStock Center | BDSC: 6314; FLYB: FBst0006314 | FLYB genotype: y1 w* P{UAS-mCD8::GFP.L}Ptp4ELL4 P{GawB}lzgal4 | |||
| Genetic reagent (D. melanogaster) | E(spl)m3-HLH-GAL4 | BloomingtonDrosophilaStock Center | BDSC: 46517; FLYB: FBst0046517 | FLYB genotype: w1118; P{GMR10E12-GAL4}attP2 | |||
| Genetic reagent (D. melanogaster) | Drip-GAL4 | BloomingtonDrosophilaStock Center | BDSC: 66782; FLYB: FBst0066782 | FLYB genotype: y1 w*; Mi{Trojan-GAL4.0}DripMI00887-TG4.0/SM6a | |||
| Genetic reagent (D. melanogaster) | btl-GAL4 | Perrimon Lab stock | NA | Genotype: yw; UAS-GFPN-lacZ(2-1)/CyO; btl-Gal4(3-1)/TM3 Sb Ser | |||
| Genetic reagent (D. melanogaster) | bnl-LexA | Dr. Sougata Roy | PMID:28502613 | bnl-LexA/TM6 | |||
| Genetic reagent (D. melanogaster) | Ubi-GAL4 | Dr. Utpal Banerjee | PMID:24267893 | Ubiquitin-GAL4 | |||
| Genetic reagent (D. melanogaster) | BcF6-mCherry | Dr. Robert Schulz | PMID:27913635 | BcF6-mCherry (III) | |||
| Genetic reagent (D. melanogaster) | msn-mCherry | Dr. Robert Schulz | PMID:27913635 | MSNF9mo-mCherry (III) | |||
| Genetic reagent (D. melanogaster) | srp-GAL4 | Dr. Lucas Waltzer | PMID:14657024 | NA | |||
| Genetic reagent (D. melanogaster) | LexAOp-myr-GFP | BloomingtonDrosophilaStock Center | BDSC:32210; FLYB: FBst0032210 |
FLYB genotype: P{13XLexAop2-IVS-myr::GFP}attP40 | |||
| Genetic reagent (D. melanogaster) | LexAOp-mCherry | BloomingtonDrosophilaStock Center | BDSC:52271; FLYB: FBst0052271 |
FLYB genotype: y1 w*; wgSp-1/CyO, P{Wee-P.ph0}BaccWee-P20; P{13XLexAop2-6XmCherry-HA}attP2 | |||
| Genetic reagent (D. melanogaster) | UAS-mCD8- GFP |
BloomingtonDrosophilaStock Center | BDSC: 5137; FLYB: FBst0005137 | FLYB genotype: y1 w*; P{UAS-mCD8::GFP.L}LL5, P{UAS-mCD8::GFP.L}2 | |||
| Genetic reagent (D. melanogaster) | UAS-poloRNAi | BloomingtonDrosophilaStock Center | BDSC: 33042; FLYB: FBst0033042 | FLYB genotype: y1 sc* v1 sev21; P{TRiP.HMS00530}attP2 | |||
| Genetic reagent (D. melanogaster) | UAS-btlRNAi-1 | BloomingtonDrosophilaStock Center | BDSC: 43544; FLYB: FBst0043544 | FLYB genotype: y1 sc* v1 sev21; P{TRiP.HMS02656}attP40 | |||
| Genetic reagent (D. melanogaster) | UAS-btlRNAi-2 | BloomingtonDrosophilaStock Center | BDSC: 60013; FLYB: FBst0060013 | FLYB genotype: y1 v1; P{TRiP.HMS05005}attP40 | |||
| Genetic reagent (D. melanogaster) | UAS-empty | Dr. Hugo Bellen | PMID:27640307 | UAS-empty (III) | |||
| Transfected construct (D. melanogaster) | NA | NA | NA | NA | |||
| Biological sample (D. melanogaster) | larval hemolymph (blood) | NA | NA | Hemocytes from hemolymph of third instar (96 and 120 hr AEL) larvae | |||
| Antibody | L1abc (mouse monoclonal) | Prof. Istvan Andó | PMID:18297797 | 1:100 dilution | |||
| Antibody | anti-PPO2 (mouse monoclonal) |
Prof. Istvan Andó | PMID:18297797 | 1:1000 dilution | |||
| Antibody | anti-Hindsight (mouse monoclonal) | Developmental Studies Hybridoma Bank | Cat# 1G9 | 1:10 dilution | |||
| Antibody | anti-Mys (mouse monoclonal) | Developmental Studies Hybridoma Bank | Cat# CF-6G11 | 1:10 dilution | |||
| Recombinant DNA reagent | NA | NA | NA | NA | |||
| Sequence-based reagent | CecC (primer) | FlyPrimerBank | PD41779 | For: GCATTGGACAATCGGAAGCC
Rev: TTGCGCAATTCCCAGTCCTT |
|||
| Sequence-based reagent | Drs (primer) | FlyPrimerBank | PD40133 | For: CTGGGACAACGAGACCTGTC
Rev: ATCCTTCGCACCAGCACTTC |
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| Sequence-based reagent | Mtk (primer) | FlyPrimerBank | PD41985 | For: GCTACATCAGTGCTGGCAGA Rev: TTAGGATTGAAGGGCGACGG | |||
| Sequence-based reagent | CG43236 (primer) | FlyPrimerBank | PD41670 | For: GCAAGAGTTTGGATGCCACC Rev: GCCTCATATCGAAAGGATTGCG | |||
| Sequence-based reagent | stg (primer) | FlyPrimerBank | PB60117 | For: GAAAACAACTGCAGCATGGAT
Rev: CGACAGCTCCTCCTGGTC |
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| Sequence-based reagent | polo (primer) | FlyPrimerBank | PP7029 | For: CCCGAGGATAAGAGCACGGA
Rev: GTCGTCGGTTTCCACATCG |
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| Sequence-based reagent | MMP1 (primer) | FlyPrimerBank | PP18419 | For: CCAGTTCGGCTATCTACCCG Rev: CTCGATGGCACTCACCCAG | |||
| Sequence-based reagent | Ance (primer) | FlyPrimerBank | PP22471 | For: GTGATACCACCAAGTTCCAATGG
Rev: GGCATAGTCGTCTTCAGGTAGAG |
|||
| Sequence-based reagent | GstE6 (primer) | FlyPrimerBank | PP10905 | For: TACGGTTTGGACCCCAGTC Rev: ATATTCCGGTGAAAGTTGGGC | |||
| Sequence-based reagent | Arc1 (primer) | FlyPrimerBank | PP10071 | For: ATGGCCCAGCTTACACAGATG Rev: GGAGAAGTTGCCTTTGCCTC | |||
| Sequence-based reagent | Prx2540-1 (primer) | FlyPrimerBank | PD40349 | For: ATGATCCTGCCCACTGTCAC Rev: CAGTGGTGCGGACGTAGTTT | |||
| Sequence-based reagent | Ubx (primer) | FlyPrimerBank | PP12922 | For: ATGAACTCGTACTTTGAACAGGC Rev: CCAGCGAGAGAGGGAATCC | |||
| Sequence-based reagent | Cpx (primer) | FlyPrimerBank | PD40622 | For: CGCGAGAAGATGAGGCAAGA Rev: CATCAGGGGATTGGGCTCTT | |||
| Sequence-based reagent | mthl7 (primer) | FlyPrimerBank | PP15001 | For: AGTTTGGGGACGGTTCGATTA Rev: TGAGACCATCATCGCATTTTCC | |||
| Sequence-based reagent | Cys (primer) | FlyPrimerBank | PP22082 | For: GGATGCCACTCTCGCACAG Rev: GGTGTTAAGACTTCCAGCTACG | |||
| Sequence-based reagent | bnl (primer) | NA | NA | For: AACCCAAATCCAATCCCAAT
Rev: GATGCTGTTGCTGTTGCTGT |
|||
| Sequence-based reagent | btl (primer) | NA | NA | For: GAGTCGATCCCTGAAGTTGC
Rev: GCAGTTGCCCCACTGTTAAT |
|||
| Sequence-based reagent | RpL32/rp49 (primer) | FlyPrimerBank | PD41810 | For: AGCATACAGGCCCAAGATCG
Rev: TGTTGTCGATACCCTTGGGC |
|||
| Peptide, recombinant protein | NA | NA | NA | NA | |||
| Commercial assay or kit | Chromium Single Cell 3’ Library and Gel Bead Kit v2 | 10x Genomics | PN-120267 | NA | |||
| Commercial assay or kit | Chromium i7 Multiplex Kit | 10x Genomics | PN-120262 | NA | |||
| Commercial assay or kit | Chromium Single Cell A Chip Kit | 10x Genomics | PN-1000009 | NA | |||
| Chemical compound, drug | NA | NA | NA | NA | |||
| Software, algorithm | Seurat | Stuart et al., 2019 | PMID:31178118 | NA | |||
| Software, algorithm | Harmony | Korsunsky et al., 2019 | PMID:31740819 | NA | |||
| Software, algorithm | Monocle 3 | Cao et al., 2019 | PMID:30787437 | NA | |||
| Software, algorithm | Jalview | Waterhouse et al., 2009 | PMID:19151095 | NA | |||
| oftware, algorithm | Biorender | https://biorender.com/ | NA | Biorender was utilized to make the schematic diagrams used in this study. | |||
| Other | DAPI (nuclear stain) | Vector Laboratories | Cat# H-1200 | Ready to use | |||
| Other | Phalloidin | ThermoFischer | Cat# A34055 | 1:100 dilution | |||
| Other | Optiprep | AxisShield | AXS-1114542 | Working concentration: 1.09 g/ml | |||
| Other | SyBr Green | Bio-Rad iQ SYBR Green Supermix | Cat# 1708880 | Working concentration: 1X | |||