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Kinetic parameters of N/H exchange in IEC-18 cells.
| vmax (nmol/mg protein•30sec) |
Km (mM) |
|
|---|---|---|
| Control | 1.9 ± 0.1 | 4.4 ± 0.3 |
| siNOS3 | 3.60 ± 0.2* | 4.2 ± 0.3 |
The maximal rate of uptake of Na (Vmax) was significantly increased in NOS3 silenced IEC-18 cells; however, the affinity (1/Km) was unchanged between control and NOS3 silenced IEC-18 cells
p < 0.01, n = 3.
