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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Mol Microbiol. 2020 Feb 11;113(5):1038–1051. doi: 10.1111/mmi.14473

Fig. 6.

Fig. 6.

TraA and MXAN_4924 are secreted by the T2SS. (A) Schematic of GspD depletion assay. CuSO4 induces GspD expression and BCS (copper chelator) blocks GspD expression. (B) Western blot shows GspD dependent secretion of TraA_ΔMXCT. BCS culture accumulates TraA_ΔMXCT in C fraction indicating a secretion block. (C) Western blot shows 4924_ΔMXCT secretion depends on GspD expression. Cells grown in BCS have reduced levels of GspD and 4924_ ΔMXCT is absent from S fraction, suggesting a secretion block made it unstable. Equal amounts of cell materials were loaded, and blots were probed with anti-FLAG, anti-GspD and anti-PilA antibodies. PilA encodes the cell surface pilus that is secreted independently of T2SS, i.e. the type IV pilus system, and serves as a negative control for the GspD depletion assay.