FIGURE 1. NZB/W F1 females acquire dispersed Xist RNA signals in naïve B cells during lupus disease development.

(A) Xist RNA FISH images for nuclei from naïve CD23+ B cells in WT-age matched controls (n = 4) and NZB/W F1 females (n = 6), for each disease category. Disease was staged using criteria described in Supplemental Figure 1. Xist RNA is shown in red, DAPI nuclear counterstain in blue. Representative cells collected from multiple independent experiments are shown. (B) Quantification of Xist RNA localization patterns in NZB/W F1 and wildtype naïve B cells for the three disease categories. The percentage of cells with Xist RNA clustered at the Xi (top), Xist RNA dispersed throughout the nuclei (middle), and with no detectable Xist RNA signals (bottom) are shown. The mean across replicates and standard deviation of the mean is shown. Significance was determined by unpaired t test. *p<0.05. Total nuclei counts for each animal are shown in Supplemental Figure 3.