FIGURE 6. Sex-specific gene expression profiling of diseased NZB/W F1 B cells reveal female-specific upregulation of autoimmunity-associated genes.
RNA from in vitro stimulated B cells from late-stage disease male and female NZB/W F1 animals was isolated for RNAseq analysis. (A) Heatmap for all differentially expressed genes (genome-wide) in B cells from female and male NZB/W F1 with late stage disease. (B) Heatmap for differentially expressed X-linked genes in female and male samples. (C) Graphs of normalized counts for some female-specific upregulated genes. (D) Graphs of normalized counts for some male-specific upregulated genes. (E) Normalized read counts of two X-linked genes. (F) Normalized counts for the X-linked gene Tlr7. Pink circles indicate female samples, blue triangles indicate male samples.