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. Author manuscript; available in PMC: 2021 May 18.
Published in final edited form as: Analyst. 2020 May 18;145(10):3517–3527. doi: 10.1039/d0an00383b

Table 2.

Effect of incubation time on ATPS ELISA (15 minutes, 1 hour and 4 hours). The performance was carried out with information, LOD, CV (%) and S/N±SD (n=3) obtaining by using each incubation time as listed below.

Incubation
time
LOD (pg mL−1) CV (%) S/N±SD ELISA image
Signal Noise
15 minutes 180 9.1% 3.70±0.03 graphic file with name nihms-1583342-t0008.jpg
1 hour ~1 1.4% 30.0±1.0 graphic file with name nihms-1583342-t0009.jpg graphic file with name nihms-1583342-t0011.jpg
4 hours 340 7.8% 1.6±0.1 graphic file with name nihms-1583342-t0010.jpg graphic file with name nihms-1583342-t0012.jpg